I may have made a mistake on a V2.5 board

I accidently applied 24v to pin 18 on the Ampseal connector on a V2.5 board, which I now see is CAN2 high. I didn’t realize on the V2.5 boards I have to run extra wires to the terminal block internally. What could I have possibly messed up?

I have done this, don’t recall any damage done, all worked fine.

Well, I have the power light on the cytron but I do not get any power out of the cytron. I pulled the teensy to reflash it, but I can’t get it to connect to my computer, the computer doesn’t recognize it. Not sure which direction to go.

Maybe your teensy have got the modification, cutting small trace from usb 5v to teensy 5v in?

I looked, it does have the modification done to cut the trace between the usb and the board. Does that mean I need to have the board powered up to flash it?

Yes either solder the cut temporary, or power it from PCB or elsewhere

So getting back to my issues, I finally found a cable that has the comm wires in it, that is why I was unable to program. I uploaded the program for v2.4 from the AOG configure software. I have a flashing orange light on the teensy. I have guidance but I have no autosteer. The cytron has 24v, the green led is on. When I enable autosteer via my switch, light and steering wheel go green on the display, I get a red light to come on the cytron for MB but never anything on MA. I get 2.4v on the output of the cytron, but no movement of the motor. I am unsure of where to go next or what I am missing.

But which colour is the roll icon just left of steering icon at button right?

green

Maybe you did not select cytron in settings AND click Send to teensy (while teensy was also connected)
Your only one side of cytron working could be ibt2 motor driver is selected?