MA and MB buttons will not require input from Teensy, check you haven’t got a bad solder joint on the Teensy.
On the pins underneath the teensy? I brought the teensy 4.1 with header pins attached and they were also in the pcb aswell but i can look 
Should i be getting any voltage on the 3 pins at the end of the cytron? Getting 0v on all 3 at standstill
In one of your pictures one of the PCB Teensy headers is longer than the Teensy!?
The Teensy outside header pins looks good.
Cytron looks like the right hand pin isn’t soldered the best.
You could test the socket though with Teensy putting a steering signal.
The ethernet pins are longer your right. Will be causing me problems?
The teensy flashes orange light on top so its getting power and the steer module light is green on the pcb
The block in the aio board is lower down so i think its good
Teensy sits down level on the header pins
Solder the middle on the cytron. It looks like not connected
Longer Ethernet pins ok, they can push into the header socket below.
I meant the female header on the PCB is longer than the Teensy, should be 24 pins long each side.
Blasted some more solder on the middle pin and tried again and still no luck.
Well spotted. I had a few bad attempts at cutting them the right length as it kept splitting the last pin out, was running out of header pins so decided to make that one longer and i pulled the last pin out to avoid damaging the pin i needed to use. 10 out of 10 for spotting that 
How do i get the teensy to put out a signal for me to test the pins on the cytron
If you have an f9p. Connect everything and fire up AOG in normal mode and make an ab line and engage autosteer. It should attempt to move your wheels to the proper steering angle but it won’t be able to. What is even better is if you setup a was so it has feedback like a real setup. For mine I use a small 12v gear motor and some string wrapped around its shaft like a winch that pulls on a WAS.
Will it do this in simulator mode? Should the motor spin in simulator mode as it tries to keep to the line?
I don’t understand why you want to use simulator mode to test the motor. Wouldn’t it be a lot easier to just use the drive feature in the steer setting? Make tractor green, make aog wheel green, and touch the left and right arrows and see if you have pwm above the green tractor button. And so on.
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Im not sure how i can test the pwm when the tractor has to be moving to get the green light on the pcb for steer module.
When stationery its all red.
Thought maybe in simulator mode when all the lights are green i could then test the cytron without the tractor actually moving
Ok i pressed the pwm tab on the screen and the light went green on the aio for steer module.
Green auto steer switch activated and tractor pointer also green.
Not getting no output on any of the pins on the rear of the cytron.
The little black 4 square tabs on the top, the front 2 are testing at 24v the rear 2 are testing at 9.4v.
Does this mean the cytron is goosed?
Get out the microscope and have a good look, that middle bottom one looks like a wizards hat rather than a soldered joint 
To test so you know what you are looking for.
With wheels fairly centered, engage the drive feature and autosteer like you have done.
click the little arrows beside the tractor button to make the error over 5v. look at the pwm amount just under the tractor button. If you have pwm over 0 under 255 then you should have voltage at pwm1 pin on the cytron. pins are ground, pwm1, and Dir. pwm1 should have something less than 3.3v when you have pwm in the steer screen. Dir should either be 5v or about 0v I don’t recall.Power for one direction, no power for the opposite direction.
I don’t recall the direction of MA/MB, but with power at dir should select either ma or ma. When you have 0v on dir the other direction shoud be enabled. so using the arrows on the steer screen, make the error go from green to red and see if ma/mb changes on the cytron. Once those are working, you should have motor output. If not then check motor power connections.
Your right i best buy a magnifying glass